Detailed information of TA system TA153419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3876674..3877299
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HFC66_RS19355
• Protein ID: WP_000911330.1
• Coordinates: 3876901..3877299 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HFC66_RS19350
• Protein ID: WP_000450524.1
• Coordinates: 3876674..3876901 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS19325	3872477..3872947	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HFC66_RS19330	3872947..3873519	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HFC66_RS19335	3873665..3874543	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HFC66_RS19340	3874560..3875594	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HFC66_RS19345	3875807..3876520	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HFC66_RS19350	3876674..3876901	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HFC66_RS19355	3876901..3877299	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HFC66_RS19360	3877446..3878309	+	864	WP_001267495.1	neutral zinc metallopeptidase	-
HFC66_RS19365	3878324..3880339	+	2016	WP_167839348.1	tRNA cytosine(34) acetyltransferase TmcA	-
HFC66_RS19370	3880413..3881111	+	699	WP_000679812.1	esterase	-
HFC66_RS19375	3881221..3881421	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T153419 WP_000911330.1 NZ_CP050865:3876901-3877299 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T153419 NZ_CP066904:328118-328486 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT153419 WP_000450524.1 NZ_CP050865:3876674-3876901 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT153419 NZ_CP066904:327900-328121 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33