Detailed information of TA system TA153417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3579212..3579795
• Replicon: chromosome
• Accession: NZ_CP050865
• Organism: Escherichia coli strain 8-3-Ti3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: HFC66_RS17845
• Protein ID: WP_000254738.1
• Coordinates: 3579460..3579795 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: HFC66_RS17840
• Protein ID: WP_000581937.1
• Coordinates: 3579212..3579460 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC66_RS17830	3575551..3576852	+	1302	WP_000046790.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HFC66_RS17835	3576900..3579134	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HFC66_RS17840	3579212..3579460	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HFC66_RS17845	3579460..3579795	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
HFC66_RS17850	3579866..3580657	+	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
HFC66_RS17855	3580885..3582522	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HFC66_RS17860	3582610..3583908	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
HFC66_RS17865	3583968..3584750	-	783	Protein_3471	YgcG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T153417 WP_000254738.1 NZ_CP050865:3579460-3579795 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T153417 NZ_CP066902:1694-2008 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT153417 WP_000581937.1 NZ_CP050865:3579212-3579460 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT153417 NZ_CP066902:1428-1706 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4