Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3469494..3470148 Replicon chromosome
Accession NZ_CP050865
Organism Escherichia coli strain 8-3-Ti3

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HFC66_RS17375 Protein ID WP_000244777.1
Coordinates 3469741..3470148 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HFC66_RS17370 Protein ID WP_000354046.1
Coordinates 3469494..3469760 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC66_RS17345 3464782..3465525 + 744 WP_000951948.1 SDR family oxidoreductase -
HFC66_RS17350 3465582..3467015 - 1434 WP_032359762.1 6-phospho-beta-glucosidase BglA -
HFC66_RS17355 3467060..3467371 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HFC66_RS17360 3467535..3468194 + 660 WP_032359756.1 hemolysin III family protein -
HFC66_RS17365 3468271..3469251 - 981 WP_032359755.1 tRNA-modifying protein YgfZ -
HFC66_RS17370 3469494..3469760 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HFC66_RS17375 3469741..3470148 + 408 WP_000244777.1 toxin CptA Toxin
HFC66_RS17380 3470188..3470709 - 522 WP_032359754.1 flavodoxin FldB -
HFC66_RS17385 3470821..3471717 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HFC66_RS17390 3471741..3472451 + 711 WP_032359753.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HFC66_RS17395 3472457..3474190 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T153416 WP_000244777.1 NZ_CP050865:3469741-3470148 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153416 NZ_CP066901:93029-93178 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153416 WP_000354046.1 NZ_CP050865:3469494-3469760 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153416 NZ_CP066901:c92984-92925 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References