Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 196955..197480 Replicon plasmid unnamed1
Accession NZ_CP050863
Organism Escherichia coli strain 8-3-DC15

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag HFC67_RS26110 Protein ID WP_001159860.1
Coordinates 197175..197480 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag HFC67_RS26105 Protein ID WP_000813626.1
Coordinates 196955..197173 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC67_RS26065 192183..192467 + 285 WP_038341659.1 ribbon-helix-helix domain-containing protein -
HFC67_RS26070 192467..192742 + 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
HFC67_RS26465 192874..193011 - 138 WP_000766699.1 hypothetical protein -
HFC67_RS26080 193118..193469 - 352 Protein_231 plasmid partitioning/stability family protein -
HFC67_RS26085 193472..194040 - 569 Protein_232 plasmid segregation protein ParM -
HFC67_RS26090 194048..195139 + 1092 Protein_233 IS66 family transposase -
HFC67_RS26095 195334..196542 - 1209 Protein_234 IS91 family transposase -
HFC67_RS26100 196523..196795 - 273 WP_000019158.1 hypothetical protein -
HFC67_RS26105 196955..197173 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HFC67_RS26110 197175..197480 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
HFC67_RS26115 197516..197611 + 96 Protein_238 transposase domain-containing protein -
HFC67_RS26120 197873..199510 + 1638 WP_039060807.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
HFC67_RS26125 199718..199987 + 270 WP_000079956.1 hypothetical protein -
HFC67_RS26470 199984..200210 + 227 Protein_241 type II toxin-antitoxin system RelE/ParE family toxin -
HFC67_RS26130 200239..200724 + 486 WP_038339729.1 protein kinase -
HFC67_RS26135 200739..201436 - 698 Protein_243 IS1 family transposase -
HFC67_RS26140 201335..201550 + 216 WP_167839519.1 hypothetical protein -
HFC67_RS26475 201560..201751 - 192 Protein_245 IS66 family insertion sequence element accessory protein TnpB -
HFC67_RS26145 201749..202073 + 325 Protein_246 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / senB / faeF / faeH / faeI / ospC1 / ospD3/senA / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..233798 233798
- inside IScluster/Tn - ospI / ipaH9.8 / ospG 180696..201242 20546


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T153406 WP_001159860.1 NZ_CP050863:197175-197480 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T153406 NZ_CP066900:742287-742772 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT153406 WP_000813626.1 NZ_CP050863:196955-197173 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT153406 NZ_CP066900:741997-742290 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References