Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 115006..115763 Replicon plasmid unnamed1
Accession NZ_CP050863
Organism Escherichia coli strain 8-3-DC15

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag HFC67_RS25620 Protein ID WP_000501969.1
Coordinates 115006..115491 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag HFC67_RS25625 Protein ID WP_011114751.1
Coordinates 115479..115763 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC67_RS25595 111128..111427 + 300 Protein_134 Tn3 family transposase -
HFC67_RS25600 111487..112284 - 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
HFC67_RS25605 112284..113456 - 1173 WP_000952382.1 IS21-like element IS21 family transposase -
HFC67_RS25610 113556..114423 + 868 Protein_137 Tn3 family transposase -
HFC67_RS25615 114498..114812 - 315 Protein_138 IS3 family transposase -
HFC67_RS25620 115006..115491 - 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
HFC67_RS25625 115479..115763 - 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
HFC67_RS25630 116665..116829 + 165 WP_001346193.1 hypothetical protein -
HFC67_RS25635 117083..118684 - 1602 WP_004967149.1 IS66-like element ISSfl3 family transposase -
HFC67_RS25640 118704..118886 - 183 WP_089519923.1 IS66 family insertion sequence element accessory protein TnpB -
HFC67_RS25645 119200..120219 - 1020 WP_000878026.1 IS110-like element ISEc11 family transposase -
HFC67_RS25650 120365..120532 - 168 Protein_145 IS66 family insertion sequence element accessory protein TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospC2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospD2 / ospF / ospD1 / ipgB2 / ipaH4.5 / ipaH7.8 / ipaH1.4 / ospE2 / senB / faeF / faeH / faeI / ospC1 / ospD3/senA / ospC2 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG 1..233798 233798
- inside IScluster/Tn - ospC1 / ospD3/senA / ospC2 / ospC3 / ipaJ 100785..129934 29149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T153404 WP_000501969.1 NZ_CP050863:c115491-115006 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T153404 NZ_CP066900:328021-328389 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT153404 WP_011114751.1 NZ_CP050863:c115763-115479 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT153404 NZ_CP066900:327803-328024 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References