Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4146224..4146855 Replicon chromosome
Accession NZ_CP050862
Organism Escherichia coli strain 8-3-DC15

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag HFC67_RS21275 Protein ID WP_001259385.1
Coordinates 4146580..4146855 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HFC67_RS21270 Protein ID WP_000593555.1
Coordinates 4146224..4146583 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC67_RS21245 4142356..4143300 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
HFC67_RS21250 4143297..4144130 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HFC67_RS21255 4144130..4144894 + 765 WP_001136223.1 nickel import ATP-binding protein NikD -
HFC67_RS21260 4144891..4145697 + 807 WP_000173703.1 nickel import ATP-binding protein NikE -
HFC67_RS21265 4145703..4146104 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HFC67_RS21270 4146224..4146583 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HFC67_RS21275 4146580..4146855 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
HFC67_RS21280 4146928..4148052 - 1125 WP_001216257.1 ABC transporter permease -
HFC67_RS21285 4148052..4150787 - 2736 WP_032359128.1 ribosome-associated ATPase/putative transporter RbbA -
HFC67_RS21290 4150784..4151851 - 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T153402 WP_001259385.1 NZ_CP050862:c4146855-4146580 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T153402 NZ_CP066898:1694-2008 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT153402 WP_000593555.1 NZ_CP050862:c4146583-4146224 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT153402 NZ_CP066898:1428-1706 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References