Detailed information of TA system TA153398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3105973..3106810
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HFC67_RS15990
• Protein ID: WP_000227784.1
• Coordinates: 3106268..3106810 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HFC67_RS15985
• Protein ID: WP_001297137.1
• Coordinates: 3105973..3106284 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS15960	3100993..3101940	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HFC67_RS15965	3101962..3103953	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HFC67_RS15970	3103943..3104557	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HFC67_RS15975	3104557..3104886	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HFC67_RS15980	3104898..3105788	+	891	WP_000971336.1	heme o synthase	-
HFC67_RS15985	3105973..3106284	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HFC67_RS15990	3106268..3106810	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HFC67_RS15995	3106866..3107306	-	441	Protein_3120	hypothetical protein	-
HFC67_RS16000	3107324..3108021	-	698	Protein_3121	IS1 family transposase	-
HFC67_RS16005	3107920..3108177	+	258	WP_167839323.1	hypothetical protein	-
HFC67_RS16010	3108077..3108577	-	501	Protein_3123	sel1 repeat family protein	-
HFC67_RS16015	3108985..3110349	+	1365	WP_032314626.1	MFS transporter	-
HFC67_RS16020	3110477..3110968	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3107324..3107827	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T153398 WP_000227784.1 NZ_CP050862:3106268-3106810 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T153398 NZ_CP066896:119668-120084 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT153398 WP_001297137.1 NZ_CP050862:3105973-3106284 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT153398 NZ_CP066896:119441-119671 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3