Detailed information of TA system TA153396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2121059..2121697
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HFC67_RS11025
• Protein ID: WP_000813794.1
• Coordinates: 2121521..2121697 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HFC67_RS11020
• Protein ID: WP_001270286.1
• Coordinates: 2121059..2121475 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS11000	2116376..2117389	-	1014	WP_032358821.1	ABC transporter ATP-binding protein	-
HFC67_RS11005	2117407..2118552	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
HFC67_RS11010	2118776..2119473	-	698	Protein_2150	IS1-like element IS1A family transposase	-
HFC67_RS11015	2119574..2120980	-	1407	WP_032358822.1	PLP-dependent aminotransferase family protein	-
HFC67_RS11020	2121059..2121475	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HFC67_RS11025	2121521..2121697	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HFC67_RS11030	2121919..2122149	+	231	WP_000494244.1	YncJ family protein	-
HFC67_RS11035	2122241..2124202	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HFC67_RS11040	2124275..2124811	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HFC67_RS11045	2124864..2126078	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2114650..2144792	30142
	-	flank	IS/Tn	-	-	2118776..2119279	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T153396 WP_000813794.1 NZ_CP050862:c2121697-2121521 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T153396 NZ_CP066895:c5406268-5405885 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT153396 WP_001270286.1 NZ_CP050862:c2121475-2121059 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT153396 NZ_CP066895:c5406510-5406268 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 6HPB