Detailed information of TA system TA153387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 709397..710022
• Replicon: chromosome
• Accession: NZ_CP050862
• Organism: Escherichia coli strain 8-3-DC15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HFC67_RS03530
• Protein ID: WP_000911330.1
• Coordinates: 709624..710022 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HFC67_RS03525
• Protein ID: WP_000450524.1
• Coordinates: 709397..709624 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFC67_RS03500	705200..705670	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HFC67_RS03505	705670..706242	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HFC67_RS03510	706388..707266	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HFC67_RS03515	707283..708317	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HFC67_RS03520	708530..709243	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HFC67_RS03525	709397..709624	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HFC67_RS03530	709624..710022	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HFC67_RS03535	710169..711032	+	864	WP_001267495.1	neutral zinc metallopeptidase	-
HFC67_RS03540	711047..713062	+	2016	WP_167839348.1	tRNA cytosine(34) acetyltransferase TmcA	-
HFC67_RS03545	713136..713834	+	699	WP_000679812.1	esterase	-
HFC67_RS03550	713944..714144	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T153387 WP_000911330.1 NZ_CP050862:709624-710022 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T153387 NZ_CP066895:353992-354339 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT153387 WP_000450524.1 NZ_CP050862:709397-709624 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT153387 NZ_CP066895:354339-354638 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33