Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 302217..302871 Replicon chromosome
Accession NZ_CP050862
Organism Escherichia coli strain 8-3-DC15

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HFC67_RS01550 Protein ID WP_000244777.1
Coordinates 302464..302871 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HFC67_RS01545 Protein ID WP_000354046.1
Coordinates 302217..302483 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HFC67_RS01520 297505..298248 + 744 WP_000951948.1 SDR family oxidoreductase -
HFC67_RS01525 298305..299738 - 1434 WP_032359762.1 6-phospho-beta-glucosidase BglA -
HFC67_RS01530 299783..300094 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HFC67_RS01535 300258..300917 + 660 WP_032359756.1 hemolysin III family protein -
HFC67_RS01540 300994..301974 - 981 WP_032359755.1 tRNA-modifying protein YgfZ -
HFC67_RS01545 302217..302483 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HFC67_RS01550 302464..302871 + 408 WP_000244777.1 toxin CptA Toxin
HFC67_RS01555 302911..303432 - 522 WP_032359754.1 flavodoxin FldB -
HFC67_RS01560 303544..304440 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HFC67_RS01565 304464..305174 + 711 WP_032359753.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HFC67_RS01570 305180..306913 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T153384 WP_000244777.1 NZ_CP050862:302464-302871 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153384 NZ_CP066892:c83783-83634 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153384 WP_000354046.1 NZ_CP050862:302217-302483 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153384 NZ_CP066892:83828-83887 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References