Detailed information of TA system TA153378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3407468..3409056
• Replicon: chromosome
• Accession: NZ_CP050858
• Organism: Klebsiella pneumoniae strain SCH6109

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486KW17
• Locus tag: HC233_RS16840
• Protein ID: WP_004190166.1
• Coordinates: 3407468..3408790 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HC233_RS16845
• Protein ID: WP_002904790.1
• Coordinates: 3408790..3409056 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC233_RS16825	3402524..3405025	+	2502	WP_167835687.1	fimbrial biogenesis outer membrane usher protein	-
HC233_RS16830	3405013..3406017	+	1005	WP_167835688.1	type 1 fimbrial protein	-
HC233_RS16835	3406159..3407451	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HC233_RS16840	3407468..3408790	-	1323	WP_004190166.1	type II toxin-antitoxin system HipA family toxin	Toxin
HC233_RS16845	3408790..3409056	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HC233_RS16850	3409321..3409647	-	327	WP_004143476.1	hydroxyisourate hydrolase	-
HC233_RS16855	3409644..3410144	-	501	WP_167835689.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HC233_RS16860	3410122..3411501	-	1380	WP_004184021.1	purine permease	-
HC233_RS16865	3411847..3413001	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HC233_RS16870	3412982..3413905	-	924	WP_004190172.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mgtC / iroE	3206176..3449600	243424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T153378 WP_004190166.1 NZ_CP050858:c3408790-3407468 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T153378 NZ_CP066891:4135725-4135943 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT153378 WP_002904790.1 NZ_CP050858:c3409056-3408790 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT153378 NZ_CP066891:4135324-4135698 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A486KW17

Antitoxin

• AlphaFold DB: A0A0H3GXD8