Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 666424..667049 Replicon chromosome
Accession NZ_CP050858
Organism Klebsiella pneumoniae strain SCH6109

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HC233_RS03245 Protein ID WP_004187928.1
Coordinates 666424..666807 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC233_RS03250 Protein ID WP_004150355.1
Coordinates 666807..667049 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC233_RS03230 663790..664692 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HC233_RS03235 664689..665324 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC233_RS03240 665321..666250 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HC233_RS03245 666424..666807 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HC233_RS03250 666807..667049 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC233_RS03255 667243..668160 + 918 WP_167835432.1 alpha/beta hydrolase -
HC233_RS03260 668174..669115 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC233_RS03265 669160..669597 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC233_RS03270 669594..670454 - 861 WP_004146232.1 virulence factor BrkB family protein -
HC233_RS03275 670448..671047 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T153365 WP_004187928.1 NZ_CP050858:c666807-666424 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153365 NZ_CP066887:c4891299-4891015 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153365 WP_004150355.1 NZ_CP050858:c667049-666807 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153365 NZ_CP066887:c4891531-4891289 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References