Detailed information of TA system TA153361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 67170..68206
• Replicon: plasmid pSsAf2.3-1
• Accession: NZ_CP050856
• Organism: Serratia symbiotica strain CWBI-2.3

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: SYMBAF_RS16710
• Protein ID: WP_040265150.1
• Coordinates: 67631..68206 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: SYMBAF_RS16705
• Protein ID: WP_040265151.1
• Coordinates: 67170..67631 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYMBAF_RS16675	62718..63170	-	453	WP_185899936.1	hypothetical protein	-
SYMBAF_RS16680	63214..63522	-	309	WP_185899943.1	DUF4935 domain-containing protein	-
SYMBAF_RS16685	64303..64710	+	408	WP_185899937.1	tyrosine-type recombinase/integrase	-
SYMBAF_RS16690	64674..65020	+	347	Protein_44	tyrosine-type recombinase/integrase	-
SYMBAF_RS16695	65136..65756	+	621	WP_040265157.1	ParA family protein	-
SYMBAF_RS16700	65805..66032	+	228	WP_040265155.1	DNA partition complex ParG	-
SYMBAF_RS16705	67170..67631	+	462	WP_040265151.1	helix-turn-helix domain-containing protein	Antitoxin
SYMBAF_RS16710	67631..68206	+	576	WP_040265150.1	PIN domain-containing protein	Toxin
SYMBAF_RS16715	68790..69452	+	663	WP_040265149.1	peptidyl-arginine deiminase	-
SYMBAF_RS16720	69545..69826	+	282	WP_040265148.1	hypothetical protein	-
SYMBAF_RS16725	70041..70682	+	642	WP_052447749.1	hypothetical protein	-
SYMBAF_RS16730	71194..72234	+	1041	WP_040265147.1	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pilW	1..82638	82638
	-	flank	IS/Tn	-	-	71194..72234	1040

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21511.90 Da
• Isoelectric Point: 4.4984

>T153361 WP_040265150.1 NZ_CP050856:67631-68206 [Serratia symbiotica]
MNYTPYPVILDACVLYPSFLRDLLIRLGLTGMYQPKWSATIEDEWQRNLLATRADLTPEQIQRTATLMNKAVPDAMIIDF
EPLIDSVDLPDVDDRHVVAAAVRSNAEIIVTFNLKDFPASALDAFGIEALHPDDFVMDMFDLNRALVLSAVTTQRSNLRK
PPMSVDGYLEALLRQGMAQTVKELGKYRLLI

Nucleotide

• Length: 576 bp

>T153361 NZ_CP066887:2033534-2033636 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17186.69 Da
• Isoelectric Point: 6.0003

>AT153361 WP_040265151.1 NZ_CP050856:67170-67631 [Serratia symbiotica]
MTNSILDSLSLPAKGEIEAAVRGQRELAAYLSTKTETQKIAIQDADNITHQIELPTSSLTLLMSILGELALGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKMRFHKTGRHRRVLFADLMEYKDQRYRESNKAMQELADLSQELELY

Nucleotide

• Length: 462 bp

>AT153361 NZ_CP066887:c2033643-2033498 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT