Detailed information of TA system TA153355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 2402317..2402940
• Replicon: chromosome
• Accession: NZ_CP050855
• Organism: Serratia symbiotica strain CWBI-2.3

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E9CPA8
• Locus tag: SYMBAF_RS12050
• Protein ID: WP_006709525.1
• Coordinates: 2402737..2402940 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: SYMBAF_RS12045
• Protein ID: WP_040266826.1
• Coordinates: 2402317..2402685 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYMBAF_RS12025	2397819..2398697	-	879	WP_040266823.1	metal ABC transporter substrate-binding protein	-
SYMBAF_RS12030	2398725..2399582	-	858	WP_040266824.1	metal ABC transporter permease	-
SYMBAF_RS12035	2399579..2400292	-	714	WP_040267096.1	ABC transporter ATP-binding protein	-
SYMBAF_RS12040	2400701..2401801	+	1101	Protein_2355	ISAs1 family transposase	-
SYMBAF_RS12045	2402317..2402685	+	369	WP_040266826.1	Hha toxicity modulator TomB	Antitoxin
SYMBAF_RS12050	2402737..2402940	+	204	WP_006709525.1	hemolysin expression modulator Hha	Toxin
SYMBAF_RS12060	2403893..2404207	+	315	WP_040266830.1	MGMT family protein	-
SYMBAF_RS12065	2404293..2405156	+	864	WP_040266834.1	acyl-CoA thioesterase II	-
SYMBAF_RS12070	2405417..2406703	-	1287	WP_040266836.1	ammonium transporter AmtB	-
SYMBAF_RS12075	2406738..2407076	-	339	WP_040266837.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	clpP	2343027..2423995	80968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8090.38 Da
• Isoelectric Point: 7.9816

>T153355 WP_006709525.1 NZ_CP050855:2402737-2402940 [Serratia symbiotica]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSNDELELFYSAADHRLAELTMNKLYDKIPTAVWKYVR

Nucleotide

• Length: 204 bp

>T153355 NZ_CP066885:787723-787944 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14310.10 Da
• Isoelectric Point: 4.3383

>AT153355 WP_040266826.1 NZ_CP050855:2402317-2402685 [Serratia symbiotica]
MDEYSSKRHDIAQLKFLCENLYDEGIATLDDSYHGWVNDPTSFVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDAYMLFSSYGINDSDLRRWQKTKAKLFRMFSGEGICTTMKT

Nucleotide

• Length: 369 bp

>AT153355 NZ_CP066885:787423-787710 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG

Structures

Toxin

• AlphaFold DB: A0A068R9C7