Detailed information of TA system TA153352

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 764561..765306
• Replicon: chromosome
• Accession: NZ_CP050855
• Organism: Serratia symbiotica strain CWBI-2.3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: SYMBAF_RS03905
• Protein ID: WP_040263016.1
• Coordinates: 764561..765052 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: SYMBAF_RS03910
• Protein ID: WP_040263014.1
• Coordinates: 765040..765306 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYMBAF_RS03885	760358..761026	-	669	WP_040263447.1	lipoyl(octanoyl) transferase LipB	-
SYMBAF_RS03890	761159..761425	-	267	WP_040263021.1	DUF493 family protein YbeD	-
SYMBAF_RS03895	761950..762659	-	710	Protein_762	IS1 family transposase	-
SYMBAF_RS03900	763119..764330	-	1212	WP_040263018.1	D-alanyl-D-alanine carboxypeptidase DacA	-
SYMBAF_RS03905	764561..765052	-	492	WP_040263016.1	GNAT family N-acetyltransferase	Toxin
SYMBAF_RS03910	765040..765306	-	267	WP_040263014.1	DUF1778 domain-containing protein	Antitoxin
SYMBAF_RS03920	766894..767663	-	770	Protein_767	endolytic peptidoglycan transglycosylase RlpA	-
SYMBAF_RS03925	767674..768786	-	1113	WP_040263012.1	peptidoglycan glycosyltransferase MrdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	633596..819570	185974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17861.71 Da
• Isoelectric Point: 9.9670

>T153352 WP_040263016.1 NZ_CP050855:c765052-764561 [Serratia symbiotica]
VGKVTAPTPLNTTHILSEFHSRETALDEWIKHQGLKNQSIGAARTFVVCREHSSQVVGFYSLATGSVTHVIAPRSLRRNM
PDPIPVIILARLAVDAGYHGKGLGADLLHDAVLRICRVAENIGVRAIMVHTLSDAAKQFYLHHGLMQSTTQEKTLFLRLP
GIV

Nucleotide

• Length: 492 bp

>T153352 NZ_CP066884:787623-787844 [Staphylococcus pseudintermedius]
ATGGAAAATGGTTATGTTAAAGTAACACAACTGACAACTGAAGCACAGCAACAACAGCTATCTGATCGTTTGCTTCGTTT
AATTGGTGTCGAGTCTGTTGCTATTGATGTGGCAACACAATCGTTGACGCTCACTTATGACACGCCAGCAAATTTGAACA
CGATAGAAAAAGAAATTTATGATGCAGGATTTCCTGTTATCCAATCACATAAAGGAGTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 4.4857

>AT153352 WP_040263014.1 NZ_CP050855:c765306-765040 [Serratia symbiotica]
MKSDVQLNIRAKEAQRVLIDTAADILHKSRTDFILEIACQAAENVLLDRRMFNLNDEQYAEFIDMLDEPVTANPALDALL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT153352 NZ_CP066884:787323-787610 [Staphylococcus pseudintermedius]
ATGACTGAACAAGCACATCATTCTGAAGAAATTAAACGTAACTTGAAGTCGCGTTTGAATCGTATTGAAGGCCAAGTGAA
AGCAATCAACCGAATGATTGACGAAGATGTCTATTGTGATGACGTGTTGACGCAAATTAGAGCGACACGTTCGGCTTTAA
ACAGTGTGGCGTCGCGTCTGTTAGATCATCATATGAAAGGATGCATCAAGCATAAGATTGACGCGGGACACCAAGACGAA
GCGATGGAAGAGTTATTAGTAACATTTCAAAAACTCATGAAAGATTAG