Detailed information of TA system TA153351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 664207..664819
• Replicon: chromosome
• Accession: NZ_CP050855
• Organism: Serratia symbiotica strain CWBI-2.3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: SYMBAF_RS03395
• Protein ID: WP_040263148.1
• Coordinates: 664640..664819 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: E9CMQ6
• Locus tag: SYMBAF_RS03390
• Protein ID: WP_006709107.1
• Coordinates: 664207..664617 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYMBAF_RS03345	659242..659530	+	289	Protein_655	IS110 family transposase	-
SYMBAF_RS03350	659774..660585	-	812	Protein_656	IS110 family transposase	-
SYMBAF_RS03365	661060..662106	-	1047	WP_040263159.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
SYMBAF_RS03370	662253..662690	+	438	WP_040263157.1	DNA polymerase III subunit psi	-
SYMBAF_RS03375	662635..663078	+	444	WP_040263155.1	ribosomal protein S18-alanine N-acetyltransferase	-
SYMBAF_RS03380	663579..663857	+	279	WP_052447629.1	Ig-like domain-containing protein	-
SYMBAF_RS03385	663869..664171	+	303	WP_152609020.1	pyocin knob domain-containing protein	-
SYMBAF_RS03390	664207..664617	-	411	WP_006709107.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SYMBAF_RS03395	664640..664819	-	180	WP_040263148.1	type II toxin-antitoxin system HicA family toxin	Toxin
SYMBAF_RS03400	665027..666616	+	1590	WP_040263146.1	peptide chain release factor 3	-
SYMBAF_RS03405	667203..667985	+	783	WP_040263144.1	TatD family hydrolase	-
SYMBAF_RS03410	668671..669450	+	780	WP_040263142.1	deoxyribose-phosphate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	633596..819570	185974

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6688.86 Da
• Isoelectric Point: 10.7334

>T153351 WP_040263148.1 NZ_CP050855:c664819-664640 [Serratia symbiotica]
MDSRNAIAMVEADGWYLVRVKGSHHQFKHPTKKGLVTVKHPQKDIPLPTLKSIKKQEGL

Nucleotide

• Length: 180 bp

>T153351 NZ_CP066884:177865-178131 [Staphylococcus pseudintermedius]
ATGGGTAATTATTCTGTTTTAATTAAGAATTCAGCCAAAAATGACTTAAAAAAAATTAAAAAGTCACTTCTTAAAGAGCA
ATTTTCAAAAATTATTGAAGTTTTAAAGATTAACCCTTATGAAGCTAGTCAATCTTTTGAGATGCTACAACCGAAGCATT
CAGCTCGATATTCAAGGCGTCTGAATCATCAACATAGAGTTGTGTATACGGTTGATGATGAAAAAAGAGAAGTTTATATT
TTTTCTGCTTGGTCACATTATGAGTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14742.72 Da
• Isoelectric Point: 4.3299

>AT153351 WP_006709107.1 NZ_CP050855:c664617-664207 [Serratia symbiotica]
MLYPVAIDKGDSSFGVRVPDIPGCFSGGDNYQDAIESVREAIDAHIELLVEDGEAVPEATSVENWLADPDYAGVVWALVD
VDVTRLMGKAEKINVTLPSLLIRRIDQFVAAHPEYGSRSGFLSRLAADKVIGRAKI

Nucleotide

• Length: 411 bp

>AT153351 NZ_CP066884:177608-177865 [Staphylococcus pseudintermedius]
ATGATTATTAAAACGCCTACAAATGCTAGAAAAGAGTTTTACGAGTTGTTAAAACAAGTTAATGATAATCATGAGCCTAT
TATAATAAGTGGTTCTAAAAGTGAAAACAATGCAGTTATTGTTAGTCAGGAAGACTGGGATAGTATTCAAGAAACGTTAT
TTCTCGAATACACTGGTACTATGGATAAAGTAAGAGCACGTGAAAAAGATGATAGTGGTTATACCAATATTGACGATATA
GATTGGGATAATTTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A068RBW1