Detailed information of TA system TA153349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 55551..56152
• Replicon: chromosome
• Accession: NZ_CP050855
• Organism: Serratia symbiotica strain CWBI-2.3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: SYMBAF_RS00300
• Protein ID: WP_040264567.1
• Coordinates: 55551..55934 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: H3R939
• Locus tag: SYMBAF_RS00305
• Protein ID: WP_006117881.1
• Coordinates: 55931..56152 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SYMBAF_RS00285	51315..52151	-	837	WP_040264570.1	GPO family capsid scaffolding protein	-
SYMBAF_RS00290	52311..54041	+	1731	WP_040264569.1	terminase family protein	-
SYMBAF_RS00295	54041..55099	+	1059	WP_040264568.1	phage portal protein	-
SYMBAF_RS00300	55551..55934	-	384	WP_040264567.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
SYMBAF_RS00305	55931..56152	-	222	WP_006117881.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SYMBAF_RS00310	56219..56915	-	697	Protein_61	DNA adenine methylase	-
SYMBAF_RS00315	57069..59453	-	2385	WP_040264566.1	replication endonuclease	-
SYMBAF_RS00320	59450..60478	-	1029	WP_040264631.1	phosphoadenosine phosphosulfate reductase family protein	-
SYMBAF_RS00325	60624..60860	-	237	WP_040264565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaD	2081..124436	122355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (4-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-59)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14070.12 Da
• Isoelectric Point: 5.5864

>T153349 WP_040264567.1 NZ_CP050855:c55934-55551 [Serratia symbiotica]
MKWISAQELIAFHDRLLAALPGVQGMPEPGRAEAIIYRVQNQLYYEGVEDIHELAAMYLVAISRGHIFNDGNKRTAFFVA
MAFLKRNGIDIRDEGNELEELTVSAAMGSLSSGDVAAVLRRLSINPH

Nucleotide

• Length: 384 bp

>T153349 NZ_CP066853:c4885441-4885187 [Rhodococcus biphenylivorans]
GTGAACATCACCTTCACCGACGAAGCCTGGGACGACTACCAGTTCTGGATCTCGACCGACAGAAAGCAGCTCGAGCGTGT
CAACGAGCTGATCAGGGATTGCCAACGAGACCCGTACTCGGGGATCGGCAAACCCGAACGGCTCAGACATGCCCTGTCAG
GACTGTGGTCTCGACGTATCGACAAAGAACACCGCCTGGTCTACGAGGTCATCGACGGTGCCCTGGTCATCATCGCCGCC
CGCTACCACTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8110.06 Da
• Isoelectric Point: 5.0644

>AT153349 WP_006117881.1 NZ_CP050855:c56152-55931 [Serratia symbiotica]
MRTYTTSQARQNIAEVMEAATAGEPVEITRRDGSAAVLISRDDFNAWQEAKLDAEFAEIMGRHGRTIKALADR

Nucleotide

• Length: 222 bp

>AT153349 NZ_CP066853:c4885686-4885438 [Rhodococcus biphenylivorans]
ATGGCTGCCATCACTGCTACCGAAGCGAGAAAGAACTTCTTTCCGCTGCTCGAACAGGTCAACAAGGACCACACCCCTGT
GGAAATCGTATCCAAGCGGGGCAATGCCGTCCTGATGTCGAAGGATGACTACGACGCCATGCTCGAGACCTCGTACCTGA
TGCAGTCTCCGGCCAACGCCCGACGACTGCTCGCCAGCATGCAGAGAGCTCGCGGAGGTGAACACACCGAAAGGGAACTC
ATCGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A068Z6U5