Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5047594..5048219 Replicon chromosome
Accession NZ_CP050845
Organism Klebsiella pneumoniae strain Bckp206

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC704_RS25060 Protein ID WP_002882817.1
Coordinates 5047594..5047977 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC704_RS25065 Protein ID WP_004150355.1
Coordinates 5047977..5048219 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC704_RS25045 5044960..5045862 + 903 WP_031590602.1 formate dehydrogenase subunit beta -
HC704_RS25050 5045859..5046494 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC704_RS25055 5046491..5047420 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HC704_RS25060 5047594..5047977 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC704_RS25065 5047977..5048219 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC704_RS25070 5048424..5049341 + 918 WP_009309725.1 alpha/beta hydrolase -
HC704_RS25075 5049355..5050296 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC704_RS25080 5050341..5050778 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC704_RS25085 5050775..5051635 - 861 WP_002882807.1 virulence factor BrkB family protein -
HC704_RS25090 5051629..5052228 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153334 WP_002882817.1 NZ_CP050845:c5047977-5047594 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153334 NZ_CP066851:206723-207208 [Salmonella enterica subsp. enterica serovar Heidelberg]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153334 WP_004150355.1 NZ_CP050845:c5048219-5047977 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153334 NZ_CP066851:206448-206735 [Salmonella enterica subsp. enterica serovar Heidelberg]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References