Detailed information of TA system TA153318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 91489..92399
• Replicon: plasmid pBckp186
• Accession: NZ_CP050844
• Organism: Klebsiella pneumoniae strain Bckp186

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HC697_RS26960
• Protein ID: WP_032413375.1
• Coordinates: 91489..91959 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: HC697_RS26965
• Protein ID: WP_032413443.1
• Coordinates: 91956..92399 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC697_RS26930	87916..88332	-	417	WP_045337409.1	type II toxin-antitoxin system VapC family toxin	-
HC697_RS26935	88329..88559	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HC697_RS26940	88813..89780	-	968	Protein_82	IS5 family transposase	-
HC697_RS26945	89903..90096	-	194	Protein_83	transposase	-
HC697_RS26950	90170..90547	+	378	Protein_84	transposase	-
HC697_RS26955	91119..91379	+	261	WP_017901409.1	hypothetical protein	-
HC697_RS26960	91489..91959	-	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
HC697_RS26965	91956..92399	-	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
HC697_RS26970	92499..93011	-	513	Protein_88	DUF4113 domain-containing protein	-
HC697_RS26975	93112..93297	-	186	WP_071571073.1	DUF2188 domain-containing protein	-
HC697_RS26980	93317..93541	+	225	Protein_90	transposase	-
HC697_RS26985	93675..93800	-	126	Protein_91	transposase	-
HC697_RS26990	93858..94205	-	348	WP_032413377.1	hypothetical protein	-
HC697_RS26995	94342..95520	-	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
HC697_RS27000	95567..96565	-	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..223022	223022
	-	inside	IScluster/Tn	-	pla	81792..93830	12038

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T153318 WP_032413375.1 NZ_CP050844:c91959-91489 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T153318 NZ_CP066844:c2467497-2467321 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT153318 WP_032413443.1 NZ_CP050844:c92399-91956 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT153318 NZ_CP066844:c2467275-2466859 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4