Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 87916..88559 Replicon plasmid pBckp186
Accession NZ_CP050844
Organism Klebsiella pneumoniae strain Bckp186

Toxin (Protein)


Gene name vagD Uniprot ID A0A443XCP1
Locus tag HC697_RS26930 Protein ID WP_045337409.1
Coordinates 87916..88332 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag HC697_RS26935 Protein ID WP_001261276.1
Coordinates 88329..88559 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC697_RS26915 83354..83836 - 483 WP_004210306.1 hypothetical protein -
HC697_RS26920 84309..85244 - 936 WP_004026564.1 omptin family outer membrane protease Kop -
HC697_RS26930 87916..88332 - 417 WP_045337409.1 type II toxin-antitoxin system VapC family toxin Toxin
HC697_RS26935 88329..88559 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HC697_RS26940 88813..89780 - 968 Protein_82 IS5 family transposase -
HC697_RS26945 89903..90096 - 194 Protein_83 transposase -
HC697_RS26950 90170..90547 + 378 Protein_84 transposase -
HC697_RS26955 91119..91379 + 261 WP_017901409.1 hypothetical protein -
HC697_RS26960 91489..91959 - 471 WP_032413375.1 RES family NAD+ phosphorylase -
HC697_RS26965 91956..92399 - 444 WP_032413443.1 DUF2384 domain-containing protein -
HC697_RS26970 92499..93011 - 513 Protein_88 DUF4113 domain-containing protein -
HC697_RS26975 93112..93297 - 186 WP_071571073.1 DUF2188 domain-containing protein -
HC697_RS26980 93317..93541 + 225 Protein_90 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..223022 223022
- inside IScluster/Tn - pla 81792..93830 12038


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15066.53 Da        Isoelectric Point: 8.5403

>T153317 WP_045337409.1 NZ_CP050844:c88332-87916 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T153317 NZ_CP066844:2076749-2076852 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT153317 WP_001261276.1 NZ_CP050844:c88559-88329 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT153317 NZ_CP066844:c2076890-2076619 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443XCP1

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References