Detailed information of TA system TA153298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 69230..69966
• Replicon: plasmid pBckp101-1
• Accession: NZ_CP050841
• Organism: Klebsiella pneumoniae strain Bckp101

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HC692_RS26650
• Protein ID: WP_003026803.1
• Coordinates: 69484..69966 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HC692_RS26645
• Protein ID: WP_003026799.1
• Coordinates: 69230..69496 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC692_RS26635	65212..65790	-	579	WP_004206571.1	recombinase family protein	-
HC692_RS26640	65949..68933	+	2985	Protein_67	Tn3 family transposase	-
HC692_RS26645	69230..69496	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HC692_RS26650	69484..69966	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HC692_RS26655	70178..71524	+	1347	WP_077251107.1	ISNCY family transposase	-
HC692_RS26660	71684..72388	+	705	WP_040167821.1	toll/interleukin-1 receptor domain-containing protein	-
HC692_RS26665	72723..72980	-	258	WP_023292103.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97812	97812
	-	inside	IScluster/Tn	-	-	65212..71524	6312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T153298 WP_003026803.1 NZ_CP050841:69484-69966 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T153298 NZ_CP066839:2910115-2910222 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT153298 WP_003026799.1 NZ_CP050841:69230-69496 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT153298 NZ_CP066839:c2910068-2910002 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66