Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5249822..5250447 Replicon chromosome
Accession NZ_CP050840
Organism Klebsiella pneumoniae strain Bckp101

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC692_RS25905 Protein ID WP_002882817.1
Coordinates 5249822..5250205 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC692_RS25910 Protein ID WP_004150355.1
Coordinates 5250205..5250447 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC692_RS25890 5247188..5248090 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HC692_RS25895 5248087..5248722 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC692_RS25900 5248719..5249648 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HC692_RS25905 5249822..5250205 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC692_RS25910 5250205..5250447 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC692_RS25915 5250652..5251569 + 918 WP_020802762.1 alpha/beta hydrolase -
HC692_RS25920 5251583..5252524 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC692_RS25925 5252569..5253006 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC692_RS25930 5253003..5253863 - 861 WP_004146232.1 virulence factor BrkB family protein -
HC692_RS25935 5253857..5254456 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153297 WP_002882817.1 NZ_CP050840:c5250205-5249822 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153297 NZ_CP066839:2909580-2909687 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153297 WP_004150355.1 NZ_CP050840:c5250447-5250205 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153297 NZ_CP066839:c2909533-2909467 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References