Detailed information of TA system TA153295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4688588..4689926
• Replicon: chromosome
• Accession: NZ_CP050840
• Organism: Klebsiella pneumoniae strain Bckp101

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: HC692_RS23220
• Protein ID: WP_167835216.1
• Coordinates: 4688588..4689538 (-)
• Length: 317 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: HC692_RS23225
• Protein ID: WP_167835217.1
• Coordinates: 4689531..4689926 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC692_RS23200	4684161..4684427	+	267	WP_032420859.1	hypothetical protein	-
HC692_RS23205	4684427..4685110	+	684	WP_031591738.1	TIGR03747 family integrating conjugative element membrane protein	-
HC692_RS23210	4685345..4686832	+	1488	WP_108978918.1	hypothetical protein	-
HC692_RS23220	4688588..4689538	-	951	WP_167835216.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HC692_RS23225	4689531..4689926	-	396	WP_167835217.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HC692_RS23230	4690001..4690834	-	834	WP_167835218.1	DUF4942 domain-containing protein	-
HC692_RS23235	4690948..4691262	-	315	WP_206030364.1	TA system toxin CbtA family protein	-
HC692_RS23240	4691311..4691628	-	318	Protein_4475	type IV toxin-antitoxin system YeeU family antitoxin	-
HC692_RS23245	4691641..4692111	-	471	WP_047361603.1	DNA repair protein RadC	-
HC692_RS23250	4692181..4693002	-	822	WP_142962278.1	DUF945 domain-containing protein	-
HC692_RS23255	4693112..4693522	-	411	WP_167835266.1	hypothetical protein	-
HC692_RS23260	4693705..4694352	-	648	WP_167835219.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4677263..4715222	37959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-304)

Antitoxin

• Protein of unknown function DUF6088 (7-114)

Sequences

Toxin

Protein

• Length: 317 a.a.
• Molecular weight: 35995.52 Da
• Isoelectric Point: 4.8742

>T153295 WP_167835216.1 NZ_CP050840:c4689538-4688588 [Klebsiella pneumoniae]
MDDLTKLFPDVADALGIESVAIVEKDYYIVELLRLLQPLAFDTHQLVFAGGTALSKAGISLNRMSEDVDIKLVPIAGFSL
QYSRNQRKNIRKGIIKTITETIVASGMFSMDEEYPKVTRDENRYNEIPVRYPQKYAQAPCLRPFIKLELMETDLLEAAEN
RPISSLVMDLTKKGEVVRAFPCATIASTQAEKLISMMRRTAASMRNVERAVDESLVRHIYDNFCIVREKGADVPVLKHFV
QECIEQDIERYGVQYPEFCESPIGELKMGLEELVNNPVYKERYLQFVTPMVFGESHVSWEEAYACFRKTVLDVVDA

Nucleotide

• Length: 951 bp

>T153295 NZ_CP066839:2611532-2612854 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14392.74 Da
• Isoelectric Point: 11.0360

>AT153295 WP_167835217.1 NZ_CP050840:c4689926-4689531 [Klebsiella pneumoniae]
MTIKARIQSRLKRSKRYVFTRDDFKDLAGYDQIGRALRELVKEGQLLKVGYGVYTKARQNVITGKLMPAAPGGSAAVVIE
ALERLKVDYRLVGATSAYNSGKSTQIPASLEIKTSPRFKRTLSVGSSKLNG

Nucleotide

• Length: 396 bp

>AT153295 NZ_CP066839:2611266-2611532 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA