Detailed information of TA system TA153294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4649309..4650119
• Replicon: chromosome
• Accession: NZ_CP050840
• Organism: Klebsiella pneumoniae strain Bckp101

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HC692_RS23030
• Protein ID: WP_065810545.1
• Coordinates: 4649309..4649842 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HC692_RS23035
• Protein ID: WP_002887278.1
• Coordinates: 4649853..4650119 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC692_RS23025	4648140..4649261	+	1122	WP_002887282.1	cupin domain-containing protein	-
HC692_RS23030	4649309..4649842	-	534	WP_065810545.1	GNAT family N-acetyltransferase	Toxin
HC692_RS23035	4649853..4650119	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HC692_RS23040	4650222..4651655	-	1434	WP_065810546.1	Cu(+)/Ag(+) sensor histidine kinase	-
HC692_RS23045	4651645..4652328	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HC692_RS23050	4652500..4653882	+	1383	WP_065810547.1	efflux transporter outer membrane subunit	-
HC692_RS23055	4653900..4654244	+	345	WP_025862174.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19770.66 Da
• Isoelectric Point: 5.2612

>T153294 WP_065810545.1 NZ_CP050840:c4649842-4649309 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPKLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGCIQLMDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T153294 NZ_CP066839:2281353-2281456 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT153294 WP_002887278.1 NZ_CP050840:c4650119-4649853 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT153294 NZ_CP066839:c2281494-2281223 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTAGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1