Detailed information of TA system TA153280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 122978..123714
• Replicon: plasmid pBckp021-3
• Accession: NZ_CP050837
• Organism: Klebsiella pneumoniae strain Bckp021

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HC680_RS29915
• Protein ID: WP_104877028.1
• Coordinates: 122978..123460 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HC680_RS29920
• Protein ID: WP_003026799.1
• Coordinates: 123448..123714 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC680_RS29895	118697..119092	-	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
HC680_RS29900	119141..120487	-	1347	WP_077253535.1	ISNCY family transposase	-
HC680_RS29905	120713..121345	+	633	WP_001567369.1	hypothetical protein	-
HC680_RS29910	121374..122777	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HC680_RS29915	122978..123460	-	483	WP_104877028.1	GNAT family N-acetyltransferase	Toxin
HC680_RS29920	123448..123714	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HC680_RS29925	123959..124393	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
HC680_RS29930	124440..124988	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
HC680_RS29935	125482..125790	+	309	WP_017896554.1	hypothetical protein	-
HC680_RS29940	127015..127506	-	492	WP_004200903.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..136867	136867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17354.02 Da
• Isoelectric Point: 9.8720

>T153280 WP_104877028.1 NZ_CP050837:c123460-122978 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T153280 NZ_CP050837:c123460-122978 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT153280 WP_003026799.1 NZ_CP050837:c123714-123448 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT153280 NZ_CP050837:c123714-123448 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66