Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 18677..19587 Replicon plasmid pBckp021-2
Accession NZ_CP050836
Organism Klebsiella pneumoniae strain Bckp021

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag HC680_RS28100 Protein ID WP_004144067.1
Coordinates 18677..19147 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag HC680_RS28105 Protein ID WP_023287184.1
Coordinates 19144..19587 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC680_RS28080 14903..15871 - 969 WP_074423483.1 IS5 family transposase -
HC680_RS28085 16176..16436 + 261 WP_017901409.1 hypothetical protein -
HC680_RS28090 16625..17593 - 969 WP_072096747.1 IS5 family transposase -
HC680_RS28095 17595..18590 - 996 WP_077254794.1 IS5 family transposase -
HC680_RS28100 18677..19147 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
HC680_RS28105 19144..19587 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
HC680_RS28110 19688..20836 - 1149 Protein_24 translesion error-prone DNA polymerase V subunit UmuC -
HC680_RS28115 20883..22370 - 1488 Protein_25 Mov34/MPN/PAD-1 family protein -
HC680_RS28120 22425..23393 - 969 WP_074424072.1 IS5 family transposase -
HC680_RS28125 23422..24171 - 750 Protein_27 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaSHV-12 - 1..237942 237942
- inside IScluster/Tn - - 1..26315 26314


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T153278 WP_004144067.1 NZ_CP050836:c19147-18677 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T153278 NZ_CP066836:2371848-2372000 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT153278 WP_023287184.1 NZ_CP050836:c19587-19144 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT153278 NZ_CP066836:c2371799-2371742 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References