Detailed information of TA system TA153276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 139749..140392
• Replicon: plasmid pBckp021-1
• Accession: NZ_CP050835
• Organism: Klebsiella pneumoniae strain Bckp021

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: HC680_RS26910
• Protein ID: WP_001044770.1
• Coordinates: 139976..140392 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HC680_RS26905
• Protein ID: WP_001261282.1
• Coordinates: 139749..139979 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC680_RS26870	135321..136187	-	867	WP_004118283.1	replication initiation protein	-
HC680_RS26875	136722..136826	+	105	WP_032409716.1	hypothetical protein	-
HC680_RS26880	136955..137212	+	258	WP_000764642.1	hypothetical protein	-
HC680_RS26885	137270..138046	-	777	WP_000015958.1	site-specific integrase	-
HC680_RS26890	138043..138786	-	744	WP_000129823.1	hypothetical protein	-
HC680_RS26895	138837..139187	-	351	WP_000493378.1	hypothetical protein	-
HC680_RS26900	139331..139792	-	462	WP_072202616.1	hypothetical protein	-
HC680_RS26905	139749..139979	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HC680_RS26910	139976..140392	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
HC680_RS26915	140466..141176	+	711	WP_004152334.1	ATP-binding protein	-
HC680_RS26920	141210..141907	+	698	Protein_173	IS1 family transposase	-
HC680_RS26925	141986..142453	+	468	Protein_174	hypothetical protein	-
HC680_RS26930	142464..142655	-	192	WP_000861580.1	hypothetical protein	-
HC680_RS26935	142664..143050	-	387	WP_001039463.1	hypothetical protein	-
HC680_RS26940	143802..144506	-	705	WP_020324562.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB6	-	1..340492	340492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T153276 WP_001044770.1 NZ_CP050835:139976-140392 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T153276 NZ_CP066836:1434248-1434634 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT153276 WP_001261282.1 NZ_CP050835:139749-139979 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT153276 NZ_CP066836:1434624-1434953 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3