Detailed information of TA system TA153275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 34986..35887
• Replicon: plasmid pBckp021-1
• Accession: NZ_CP050835
• Organism: Klebsiella pneumoniae strain Bckp021

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HC680_RS26310
• Protein ID: WP_008786589.1
• Coordinates: 34986..35444 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HC680_RS26315
• Protein ID: WP_008786588.1
• Coordinates: 35441..35887 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC680_RS26265	30712..30954	+	243	WP_007372291.1	hypothetical protein	-
HC680_RS26270	30965..31363	+	399	WP_007372292.1	hypothetical protein	-
HC680_RS26275	31447..31773	+	327	WP_007372293.1	hypothetical protein	-
HC680_RS26280	32136..32474	-	339	WP_007372294.1	hypothetical protein	-
HC680_RS26285	32712..33155	+	444	WP_007372295.1	hypothetical protein	-
HC680_RS26290	33274..33615	+	342	WP_007372296.1	hypothetical protein	-
HC680_RS26295	33774..34328	+	555	WP_007372297.1	hypothetical protein	-
HC680_RS26300	34378..34644	+	267	WP_007372298.1	hypothetical protein	-
HC680_RS26305	34712..34942	-	231	WP_007372299.1	hypothetical protein	-
HC680_RS26310	34986..35444	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HC680_RS26315	35441..35887	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HC680_RS26320	36036..36353	+	318	WP_016241555.1	hypothetical protein	-
HC680_RS26325	36395..37237	+	843	WP_007372303.1	hypothetical protein	-
HC680_RS26330	37209..37487	+	279	WP_007372304.1	hypothetical protein	-
HC680_RS26335	37547..37990	+	444	WP_007372305.1	hypothetical protein	-
HC680_RS26340	38277..38441	+	165	WP_007372306.1	hypothetical protein	-
HC680_RS26345	39009..39851	+	843	WP_007372307.1	HNH endonuclease	-
HC680_RS26350	40273..40524	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB6	-	1..340492	340492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T153275 WP_008786589.1 NZ_CP050835:c35444-34986 [Klebsiella pneumoniae]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T153275 NZ_CP066836:c1304777-1304394 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT153275 WP_008786588.1 NZ_CP050835:c35887-35441 [Klebsiella pneumoniae]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT153275 NZ_CP066836:c1305234-1304854 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: S1EZ83