Detailed information of TA system TA153274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 27686..28236
• Replicon: plasmid pBckp021-1
• Accession: NZ_CP050835
• Organism: Klebsiella pneumoniae strain Bckp021

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: HC680_RS26240
• Protein ID: WP_007372286.1
• Coordinates: 27928..28236 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: HC680_RS26235
• Protein ID: WP_007372285.1
• Coordinates: 27686..27925 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC680_RS26190	22693..23088	+	396	WP_024196081.1	hypothetical protein	-
HC680_RS26195	23270..23461	+	192	WP_016241560.1	hypothetical protein	-
HC680_RS26200	23592..24287	+	696	WP_007372278.1	hypothetical protein	-
HC680_RS26205	24310..24507	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
HC680_RS26210	24560..25018	+	459	WP_008786599.1	hypothetical protein	-
HC680_RS26215	25073..25306	+	234	WP_008786598.1	hypothetical protein	-
HC680_RS26220	25322..25954	+	633	WP_007372282.1	hypothetical protein	-
HC680_RS26225	26415..27086	+	672	WP_007372283.1	hypothetical protein	-
HC680_RS30100	27083..27547	+	465	WP_008786596.1	hypothetical protein	-
HC680_RS26235	27686..27925	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HC680_RS26240	27928..28236	+	309	WP_007372286.1	CcdB family protein	Toxin
HC680_RS26245	28257..28415	+	159	WP_016241558.1	hypothetical protein	-
HC680_RS26250	28571..29125	+	555	WP_007372288.1	hypothetical protein	-
HC680_RS26255	29195..29812	+	618	WP_007372289.1	hypothetical protein	-
HC680_RS26260	29895..30365	+	471	WP_007372290.1	hypothetical protein	-
HC680_RS26265	30712..30954	+	243	WP_007372291.1	hypothetical protein	-
HC680_RS26270	30965..31363	+	399	WP_007372292.1	hypothetical protein	-
HC680_RS26275	31447..31773	+	327	WP_007372293.1	hypothetical protein	-
HC680_RS26280	32136..32474	-	339	WP_007372294.1	hypothetical protein	-
HC680_RS26285	32712..33155	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / qnrB6	-	1..340492	340492

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T153274 WP_007372286.1 NZ_CP050835:27928-28236 [Klebsiella pneumoniae]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T153274 NZ_CP066836:1111185-1111679 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT153274 WP_007372285.1 NZ_CP050835:27686-27925 [Klebsiella pneumoniae]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT153274 NZ_CP066836:1110895-1111188 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3