Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5141617..5142242 Replicon chromosome
Accession NZ_CP050834
Organism Klebsiella pneumoniae strain Bckp021

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC680_RS25655 Protein ID WP_002882817.1
Coordinates 5141617..5142000 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC680_RS25660 Protein ID WP_004150355.1
Coordinates 5142000..5142242 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC680_RS25640 5138983..5139885 + 903 WP_004901686.1 formate dehydrogenase subunit beta -
HC680_RS25645 5139882..5140517 + 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
HC680_RS25650 5140514..5141443 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HC680_RS25655 5141617..5142000 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC680_RS25660 5142000..5142242 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC680_RS25665 5142447..5143364 + 918 WP_004901674.1 alpha/beta hydrolase -
HC680_RS25670 5143378..5144319 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC680_RS25675 5144364..5144801 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC680_RS25680 5144798..5145658 - 861 WP_002882807.1 virulence factor BrkB family protein -
HC680_RS25685 5145652..5146251 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153273 WP_002882817.1 NZ_CP050834:c5142000-5141617 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153273 NZ_CP066836:944274-944381 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153273 WP_004150355.1 NZ_CP050834:c5142242-5142000 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153273 NZ_CP066836:c944221-944160 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References