Detailed information of TA system TA153253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24078..24721
• Replicon: plasmid pBckp067
• Accession: NZ_CP050830
• Organism: Klebsiella pneumoniae strain Bckp067

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A443XCP1
• Locus tag: HC709_RS26615
• Protein ID: WP_045337409.1
• Coordinates: 24305..24721 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HC709_RS26610
• Protein ID: WP_001261276.1
• Coordinates: 24078..24308 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC709_RS26565	19096..19320	-	225	Protein_20	transposase	-
HC709_RS26570	19340..19525	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
HC709_RS26575	19626..20138	+	513	Protein_22	DUF4113 domain-containing protein	-
HC709_RS26580	20238..20681	+	444	WP_032413443.1	DUF2384 domain-containing protein	-
HC709_RS26585	20678..21148	+	471	WP_032413375.1	RES family NAD+ phosphorylase	-
HC709_RS26590	21258..21518	-	261	WP_017901409.1	hypothetical protein	-
HC709_RS26595	22090..22467	-	378	Protein_26	transposase	-
HC709_RS26600	22541..22734	+	194	Protein_27	transposase	-
HC709_RS26605	22857..23824	+	968	Protein_28	IS5 family transposase	-
HC709_RS26610	24078..24308	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HC709_RS26615	24305..24721	+	417	WP_045337409.1	type II toxin-antitoxin system VapC family toxin	Toxin
HC709_RS26620	25208..26328	+	1121	WP_088765928.1	IS3 family transposase	-
HC709_RS26625	27393..28328	+	936	WP_004026564.1	omptin family outer membrane protease Kop	-
HC709_RS26630	28801..29283	+	483	WP_004210306.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..222783	222783
	-	inside	IScluster/Tn	-	-	18807..26328	7521

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15066.53 Da
• Isoelectric Point: 8.5403

>T153253 WP_045337409.1 NZ_CP050830:24305-24721 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T153253 NZ_CP066812:c3346110-3345649 [Mycobacterium avium subsp. paratuberculosis]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAAGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGGATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT153253 WP_001261276.1 NZ_CP050830:24078-24308 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT153253 NZ_CP066812:c3346302-3346114 [Mycobacterium avium subsp. paratuberculosis]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAGAAGACCCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Toxin

• AlphaFold DB: A0A443XCP1

Antitoxin

• AlphaFold DB: A0A387K023