Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5240235..5240860 Replicon chromosome
Accession NZ_CP050829
Organism Klebsiella pneumoniae strain Bckp067

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC709_RS26075 Protein ID WP_002882817.1
Coordinates 5240235..5240618 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC709_RS26080 Protein ID WP_004150355.1
Coordinates 5240618..5240860 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC709_RS26060 5237601..5238503 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HC709_RS26065 5238500..5239135 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC709_RS26070 5239132..5240061 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HC709_RS26075 5240235..5240618 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC709_RS26080 5240618..5240860 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC709_RS26085 5241065..5241982 + 918 WP_004187175.1 alpha/beta hydrolase -
HC709_RS26090 5241996..5242937 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC709_RS26095 5242982..5243419 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC709_RS26100 5243416..5244276 - 861 WP_004146232.1 virulence factor BrkB family protein -
HC709_RS26105 5244270..5244869 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153251 WP_002882817.1 NZ_CP050829:c5240618-5240235 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153251 NZ_CP066812:1975887-1976294 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153251 WP_004150355.1 NZ_CP050829:c5240860-5240618 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153251 NZ_CP066812:1975630-1975890 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References