Detailed information of TA system TA153250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4909952..4911633
• Replicon: chromosome
• Accession: NZ_CP050829
• Organism: Klebsiella pneumoniae strain Bckp067

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: HC709_RS24500
• Protein ID: WP_004146663.1
• Coordinates: 4909952..4910869 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: HC709_RS24505
• Protein ID: WP_004177809.1
• Coordinates: 4910875..4911633 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC709_RS24480	4905258..4906778	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
HC709_RS24485	4906775..4907773	+	999	WP_002885172.1	ribose ABC transporter permease	-
HC709_RS24490	4907804..4908745	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
HC709_RS24495	4908820..4909827	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
HC709_RS24500	4909952..4910869	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HC709_RS24505	4910875..4911633	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HC709_RS24510	4911719..4912447	-	729	WP_004177810.1	response regulator transcription factor	-
HC709_RS24515	4912720..4914867	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
HC709_RS24520	4915329..4916015	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T153250 WP_004146663.1 NZ_CP050829:c4910869-4909952 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T153250 NZ_CP066812:1195364-1195735 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT153250 WP_004177809.1 NZ_CP050829:c4911633-4910875 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT153250 NZ_CP066812:1195171-1195377 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05