Detailed information of TA system TA153234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1492647..1493383
• Replicon: chromosome
• Accession: NZ_CP050829
• Organism: Klebsiella pneumoniae strain Bckp067

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HC709_RS07525
• Protein ID: WP_032429294.1
• Coordinates: 1492901..1493383 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HC709_RS07520
• Protein ID: WP_003026799.1
• Coordinates: 1492647..1492913 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC709_RS07485	1488491..1488853	-	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-
HC709_RS07490	1488905..1489255	-	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HC709_RS07495	1489613..1489861	+	249	WP_004193994.1	hypothetical protein	-
HC709_RS07500	1489858..1491069	+	1212	WP_032415728.1	hypothetical protein	-
HC709_RS07505	1491203..1491694	+	492	WP_004206662.1	hypothetical protein	-
HC709_RS07510	1491755..1491958	+	204	WP_004206661.1	hemolysin expression modulator Hha	-
HC709_RS07515	1491972..1492202	+	231	WP_004206660.1	hypothetical protein	-
HC709_RS07520	1492647..1492913	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HC709_RS07525	1492901..1493383	+	483	WP_032429294.1	GNAT family N-acetyltransferase	Toxin
HC709_RS07530	1493745..1494344	-	600	WP_032432064.1	helix-turn-helix transcriptional regulator	-
HC709_RS07535	1494557..1495501	-	945	WP_077256325.1	fimbrial protein	-
HC709_RS07540	1495513..1496091	-	579	WP_032432061.1	type 1 fimbrial protein	-
HC709_RS07545	1496095..1496835	-	741	WP_048978466.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17368.00 Da
• Isoelectric Point: 9.5822

>T153234 WP_032429294.1 NZ_CP050829:1492901-1493383 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAAFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T153234 NZ_CP066808:140528-140878 [Escherichia coli]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT153234 WP_003026799.1 NZ_CP050829:1492647-1492913 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT153234 NZ_CP066808:140883-141185 [Escherichia coli]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66