Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 146665..147575 Replicon plasmid pBckp212-1
Accession NZ_CP050827
Organism Klebsiella pneumoniae strain Bckp212

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag HC649_RS26600 Protein ID WP_004144067.1
Coordinates 147105..147575 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag HC649_RS26595 Protein ID WP_023287184.1
Coordinates 146665..147108 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC649_RS26580 141963..143144 + 1182 WP_023287181.1 hypothetical protein -
HC649_RS26585 143141..145369 + 2229 WP_023287182.1 Mov34/MPN/PAD-1 family protein -
HC649_RS26590 145416..146564 + 1149 Protein_143 translesion error-prone DNA polymerase V subunit UmuC -
HC649_RS26595 146665..147108 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
HC649_RS26600 147105..147575 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
HC649_RS26605 147662..148630 + 969 WP_000654811.1 IS5 family transposase -
HC649_RS26610 149271..150640 + 1370 WP_085956500.1 IS3-like element ISKpn8 family transposase -
HC649_RS26615 150637..150918 - 282 Protein_148 IS481 family transposase -
HC649_RS26620 150909..151877 + 969 WP_167813129.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3'')-Ib / aph(6)-Id cheD 1..186278 186278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T153230 WP_004144067.1 NZ_CP050827:147105-147575 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T153230 NZ_CP066806:c4466282-4466109 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT153230 WP_023287184.1 NZ_CP050827:146665-147108 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT153230 NZ_CP066806:c4466606-4466310 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References