Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5126176..5126801 Replicon chromosome
Accession NZ_CP050826
Organism Klebsiella pneumoniae strain Bckp212

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC649_RS25470 Protein ID WP_002882817.1
Coordinates 5126176..5126559 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC649_RS25475 Protein ID WP_004150355.1
Coordinates 5126559..5126801 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC649_RS25455 5123542..5124444 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HC649_RS25460 5124441..5125076 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC649_RS25465 5125073..5126002 + 930 WP_046654801.1 formate dehydrogenase accessory protein FdhE -
HC649_RS25470 5126176..5126559 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC649_RS25475 5126559..5126801 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC649_RS25480 5127006..5127923 + 918 WP_004181612.1 alpha/beta hydrolase -
HC649_RS25485 5127937..5128878 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC649_RS25490 5128923..5129360 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC649_RS25495 5129357..5130217 - 861 WP_046654802.1 virulence factor BrkB family protein -
HC649_RS25500 5130211..5130810 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153228 WP_002882817.1 NZ_CP050826:c5126559-5126176 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153228 NZ_CP066806:c4350304-4350014 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153228 WP_004150355.1 NZ_CP050826:c5126801-5126559 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153228 NZ_CP066806:c4350536-4350294 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References