Detailed information of TA system TA153227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4653181..4653697
• Replicon: chromosome
• Accession: NZ_CP050826
• Organism: Klebsiella pneumoniae strain Bckp212

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HC649_RS23175
• Protein ID: WP_040216106.1
• Coordinates: 4653181..4653465 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: HC649_RS23180
• Protein ID: WP_002886901.1
• Coordinates: 4653455..4653697 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC649_RS23150	4648598..4648906	-	309	WP_012737232.1	PTS sugar transporter subunit IIB	-
HC649_RS23155	4648991..4649164	+	174	WP_004146781.1	hypothetical protein	-
HC649_RS23160	4649167..4649910	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
HC649_RS23165	4650267..4652405	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
HC649_RS23170	4652713..4653177	+	465	WP_032418146.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HC649_RS23175	4653181..4653465	-	285	WP_040216106.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HC649_RS23180	4653455..4653697	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HC649_RS23185	4653775..4655685	-	1911	WP_040216105.1	BglG family transcription antiterminator	-
HC649_RS23190	4655708..4656862	-	1155	WP_002886900.1	lactonase family protein	-
HC649_RS23195	4656929..4657669	-	741	WP_004186692.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11097.94 Da
• Isoelectric Point: 10.4962

>T153227 WP_040216106.1 NZ_CP050826:c4653465-4653181 [Klebsiella pneumoniae]
MTYELAFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T153227 NZ_CP066806:c4274172-4273795 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGTCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT153227 WP_002886901.1 NZ_CP050826:c4653697-4653455 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT153227 NZ_CP066806:c4274630-4274262 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0