Detailed information of TA system TA153225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4514129..4514939
• Replicon: chromosome
• Accession: NZ_CP050826
• Organism: Klebsiella pneumoniae strain Bckp212

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HC649_RS22565
• Protein ID: WP_048337996.1
• Coordinates: 4514129..4514662 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HC649_RS22570
• Protein ID: WP_002887278.1
• Coordinates: 4514673..4514939 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC649_RS22560	4512960..4514081	+	1122	WP_004214138.1	cupin domain-containing protein	-
HC649_RS22565	4514129..4514662	-	534	WP_048337996.1	GNAT family N-acetyltransferase	Toxin
HC649_RS22570	4514673..4514939	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HC649_RS22575	4515042..4516475	-	1434	WP_048291165.1	Cu(+)/Ag(+) sensor histidine kinase	-
HC649_RS22580	4516465..4517148	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HC649_RS22585	4517320..4518705	+	1386	WP_047694163.1	efflux transporter outer membrane subunit	-
HC649_RS22590	4518723..4519067	+	345	WP_094833307.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19824.68 Da
• Isoelectric Point: 5.2614

>T153225 WP_048337996.1 NZ_CP050826:c4514662-4514129 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSLQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T153225 NZ_CP066806:c4097792-4097478 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTAG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGATAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT153225 WP_002887278.1 NZ_CP050826:c4514939-4514673 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT153225 NZ_CP066806:c4098028-4097795 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGAAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1