Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3919616..3920235 Replicon chromosome
Accession NZ_CP050826
Organism Klebsiella pneumoniae strain Bckp212

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HC649_RS19660 Protein ID WP_002892050.1
Coordinates 3920017..3920235 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HC649_RS19655 Protein ID WP_002892066.1
Coordinates 3919616..3919990 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC649_RS19645 3914768..3915961 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HC649_RS19650 3915984..3919130 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HC649_RS19655 3919616..3919990 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HC649_RS19660 3920017..3920235 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HC649_RS19665 3920394..3920960 + 567 WP_002892030.1 maltose O-acetyltransferase -
HC649_RS19670 3920932..3921072 - 141 WP_004147370.1 hypothetical protein -
HC649_RS19675 3921093..3921563 + 471 WP_048337480.1 YlaC family protein -
HC649_RS19680 3921538..3922989 - 1452 WP_048337479.1 PLP-dependent aminotransferase family protein -
HC649_RS19685 3923090..3923788 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HC649_RS19690 3923785..3923925 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HC649_RS19695 3923925..3924188 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T153224 WP_002892050.1 NZ_CP050826:3920017-3920235 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T153224 NZ_CP066806:c3894987-3894589 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT153224 WP_002892066.1 NZ_CP050826:3919616-3919990 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT153224 NZ_CP066806:c3895283-3894990 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References