Detailed information of TA system TA153222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2542014..2543602
• Replicon: chromosome
• Accession: NZ_CP050826
• Organism: Klebsiella pneumoniae strain Bckp212

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HC649_RS12760
• Protein ID: WP_048337706.1
• Coordinates: 2542014..2543336 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HC649_RS12765
• Protein ID: WP_002904790.1
• Coordinates: 2543336..2543602 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC649_RS12745	2537070..2539571	+	2502	WP_048337707.1	fimbrial biogenesis outer membrane usher protein	-
HC649_RS12750	2539559..2540563	+	1005	WP_048265557.1	type 1 fimbrial protein	-
HC649_RS12755	2540705..2541997	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HC649_RS12760	2542014..2543336	-	1323	WP_048337706.1	type II toxin-antitoxin system HipA family toxin	Toxin
HC649_RS12765	2543336..2543602	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HC649_RS12770	2543868..2544194	-	327	WP_048265555.1	hydroxyisourate hydrolase	-
HC649_RS12775	2544191..2544691	-	501	WP_048337705.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HC649_RS12780	2544669..2546048	-	1380	WP_048337704.1	purine permease	-
HC649_RS12785	2546394..2547548	+	1155	WP_048337703.1	FAD-dependent urate hydroxylase HpxO	-
HC649_RS12790	2547529..2548452	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49127.41 Da
• Isoelectric Point: 7.5357

>T153222 WP_048337706.1 NZ_CP050826:c2543336-2542014 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDVISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T153222 NZ_CP066806:3695018-3695236 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT153222 WP_002904790.1 NZ_CP050826:c2543602-2543336 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT153222 NZ_CP066806:3694618-3694992 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8