Detailed information of TA system TA153213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1735422..1736012
• Replicon: chromosome
• Accession: NZ_CP050826
• Organism: Klebsiella pneumoniae strain Bckp212

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: HC649_RS08560
• Protein ID: WP_023341911.1
• Coordinates: 1735680..1736012 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: HC649_RS08555
• Protein ID: WP_000288812.1
• Coordinates: 1735422..1735679 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC649_RS08530	1730458..1731294	+	837	WP_004180471.1	alpha/beta hydrolase	-
HC649_RS08535	1731501..1732472	+	972	WP_002911798.1	sensor domain-containing diguanylate cyclase	-
HC649_RS08540	1732469..1733572	-	1104	WP_167812951.1	phosphotransferase	-
HC649_RS26895	1733642..1733884	-	243	WP_114263991.1	hypothetical protein	-
HC649_RS08550	1734288..1735268	-	981	WP_000019441.1	IS5-like element ISKpn26 family transposase	-
HC649_RS08555	1735422..1735679	+	258	WP_000288812.1	antitoxin	Antitoxin
HC649_RS08560	1735680..1736012	+	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HC649_RS08575	1736334..1737770	+	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
HC649_RS08585	1738136..1739590	-	1455	WP_004148975.1	AMP nucleosidase	-
HC649_RS08590	1739720..1739965	-	246	WP_023284006.1	signal transduction protein PmrD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1734288..1735268	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T153213 WP_023341911.1 NZ_CP050826:1735680-1736012 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T153213 NZ_CP066806:1030160-1030567 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT153213 WP_000288812.1 NZ_CP050826:1735422-1735679 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT153213 NZ_CP066806:1029913-1030179 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0