Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 81579..82489 Replicon plasmid pBckp091-1
Accession NZ_CP050823
Organism Klebsiella pneumoniae strain Bckp091

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag HC645_RS26005 Protein ID WP_004144067.1
Coordinates 81579..82049 (-) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag HC645_RS26010 Protein ID WP_023287184.1
Coordinates 82046..82489 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC645_RS25975 76606..76818 - 213 WP_004181742.1 hypothetical protein -
HC645_RS25980 77795..78109 + 315 WP_032435789.1 hypothetical protein -
HC645_RS25990 79417..79599 - 183 Protein_80 IS5/IS1182 family transposase -
HC645_RS25995 79601..80596 - 996 WP_167803475.1 IS5 family transposase -
HC645_RS26000 80629..81492 - 864 Protein_82 IS5 family transposase -
HC645_RS26005 81579..82049 - 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
HC645_RS26010 82046..82489 - 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
HC645_RS26015 82590..83738 - 1149 Protein_85 translesion error-prone DNA polymerase V subunit UmuC -
HC645_RS26020 83785..86013 - 2229 WP_032430744.1 Mov34/MPN/PAD-1 family protein -
HC645_RS26025 86010..87191 - 1182 WP_023287181.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..157903 157903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T153208 WP_004144067.1 NZ_CP050823:c82049-81579 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T153208 NZ_CP066806:232248-232355 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT153208 WP_023287184.1 NZ_CP050823:c82489-82046 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT153208 NZ_CP066806:c232199-232136 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References