Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5109375..5110000 Replicon chromosome
Accession NZ_CP050822
Organism Klebsiella pneumoniae strain Bckp091

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HC645_RS25195 Protein ID WP_002882817.1
Coordinates 5109375..5109758 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HC645_RS25200 Protein ID WP_004150355.1
Coordinates 5109758..5110000 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC645_RS25180 5106741..5107643 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HC645_RS25185 5107640..5108275 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HC645_RS25190 5108272..5109201 + 930 WP_117244346.1 formate dehydrogenase accessory protein FdhE -
HC645_RS25195 5109375..5109758 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HC645_RS25200 5109758..5110000 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HC645_RS25205 5110205..5111122 + 918 WP_117244345.1 alpha/beta hydrolase -
HC645_RS25210 5111136..5112077 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HC645_RS25215 5112122..5112559 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HC645_RS25220 5112556..5113416 - 861 WP_004146232.1 virulence factor BrkB family protein -
HC645_RS25225 5113410..5114009 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T153207 WP_002882817.1 NZ_CP050822:c5109758-5109375 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T153207 NZ_CP066806:231764-231871 [Escherichia coli]
ATGACGCTCGCAGCGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT153207 WP_004150355.1 NZ_CP050822:c5110000-5109758 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT153207 NZ_CP066806:c231715-231652 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References