Detailed information of TA system TA153190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 219522..220261
• Replicon: chromosome
• Accession: NZ_CP050822
• Organism: Klebsiella pneumoniae strain Bckp091

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HC645_RS01105
• Protein ID: WP_021312536.1
• Coordinates: 219776..220261 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HC645_RS01100
• Protein ID: WP_003026799.1
• Coordinates: 219522..219788 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC645_RS01085	215025..217094	+	2070	WP_167803348.1	glycine--tRNA ligase subunit beta	-
HC645_RS01090	217391..218760	+	1370	WP_167803349.1	IS3 family transposase	-
HC645_RS01095	218961..219389	+	429	WP_004901287.1	GFA family protein	-
HC645_RS01100	219522..219788	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HC645_RS01105	219776..220261	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
HC645_RS01110	220605..220757	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
HC645_RS01115	221059..222678	+	1620	WP_167803350.1	ATP-binding cassette domain-containing protein	-
HC645_RS01120	222777..222989	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
HC645_RS01125	223242..223532	-	291	WP_167803351.1	HTH-type transcriptional regulator	-
HC645_RS01130	223778..225133	-	1356	WP_004901270.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	218035..218760	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T153190 WP_021312536.1 NZ_CP050822:219776-220261 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T153190 NZ_CP066796:471730-472005 [Erwinia amylovora]
ATGGAAATACAGTGGACCCAAAAGGCCCAGGATGACCTGGAGCGTATTTATAGGTTTGCCTGTCAGTACAGTCGGCAACA
TGCTGACAACGTATTAGAGCGTTTGCTCATAGGAACCTCAGGGCTCGCCGACCATCCAAGAATCGGTGTTTCTCAGATAA
GATATGAACCACGAGAAGTGAGAAAAATCTTGTTTGATGATTATGACGTTCACTATGAAATTCAGCGCGACACTATCTAT
ATTGTTGACCTTTGGCATACCCGAGAGGATCGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT153190 WP_003026799.1 NZ_CP050822:219522-219788 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT153190 NZ_CP066796:471478-471726 [Erwinia amylovora]
ATGAGACAACTCGCGAAACAGACGATCAGCGCTCAGATCCCTGCAGAGCTTGCCGCGGCGGTTGAAAACCTGGCTATTGA
ACTGGACAGATCTAAAAGCTGGGTGATTAAAGAAGCGCTGACCGCCATGATTGAAGAGAGAGAGCGGCGGCATCAGCAGA
TTTTGAAAGGGCTTGCTGATGTCGATGCGGGAAGAGTGGTTAGCCATGCGGATGTAGTTGACTTCGCTAACAAACTGAAA
AAATCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66