Detailed information of TA system TA153189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 15220..16846
• Replicon: plasmid pYRW13-131
• Accession: NZ_CP050813
• Organism: Yokenella regensburgei strain W13

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HEC60_RS24780
• Protein ID: WP_167810237.1
• Coordinates: 15521..16846 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HEC60_RS24775
• Protein ID: WP_167810236.1
• Coordinates: 15220..15516 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC60_RS24740	10580..10732	-	153	Protein_11	IS3 family transposase	-
HEC60_RS24745	10819..11556	+	738	WP_167810233.1	IS21-like element helper ATPase IstB	-
HEC60_RS24750	11734..12438	+	705	Protein_13	IS6 family transposase	-
HEC60_RS24755	12446..13342	-	897	WP_167810234.1	DUF4158 domain-containing protein	-
HEC60_RS24760	13493..14074	+	582	WP_167810235.1	recombinase family protein	-
HEC60_RS24770	14287..14990	-	704	Protein_16	IS6 family transposase	-
HEC60_RS24775	15220..15516	+	297	WP_167810236.1	helix-turn-helix transcriptional regulator	Antitoxin
HEC60_RS24780	15521..16846	+	1326	WP_167810237.1	type II toxin-antitoxin system HipA family toxin	Toxin
HEC60_RS24785	16980..17683	+	704	Protein_19	IS6 family transposase	-
HEC60_RS24790	17979..18632	-	654	WP_167810238.1	hypothetical protein	-
HEC60_RS24795	19031..19982	-	952	Protein_21	transposase	-
HEC60_RS24800	20585..21193	-	609	Protein_22	fimbrial chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	prgI / spaP / invC/sctN / invA	1..131292	131292
	-	inside	IScluster/Tn	-	-	626..17683	17057

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-405)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50669.81 Da
• Isoelectric Point: 6.1549

>T153189 WP_167810237.1 NZ_CP050813:15521-16846 [Yokenella regensburgei]
MSRRQQRLAIWMNGIHVGFWEKSKGEDSLQYLPEWSADEHGRPLSLSLPFTPGDQILRGNVVRNYFDNLLPDSEGIRRRL
AMLYQAESLEPFDLLTELGKDCVGAIQLLNSDEEPNDLYSVKFRPLSEPEIATILRNTTEVLLPGRRDVTDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSTSVENEWLCSLLLEQYGFSIAKTHIAQFEDQKALVIKRF
DRKWSADRQWIVRLPQEDMCQALGVSPLRKYQADGGPGISEIMEILSYSDHAEQDRMHFFRAQIIFWLMAATDGHAKNFS
IAIEPQGRYHLTPLYDVLSAWPVIGHGNNQIAWQKCKLAMAVRGSSNYYHICRIQKRHWIKHGESTGLSKQQVETMIDEV
ICETPSVIERVYTQLPDQFPQELAESVFEGMRHQCKRLTEK

Nucleotide

• Length: 1326 bp

>T153189 NZ_CP066796:470115-470435 [Erwinia amylovora]
ATGCACATTTCAACTGTACCGGCCACGGTGCCGGTTTCGTCACGCCTGTCTCCCGTGCAGGTGTGGCAGCAACTTTTAAC
GTATCTGCTGGAGCATCATTACGGCTTGACACTAAATGACACGCCATTCCATGACGACGCGGCCATTGAGGAGCATATCG
ACGCAGGAATCACGCTCGCCGATGCTGTTAATTTTCTGGTGGAACGTTATGAACTGGTACGTATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCCTGGCCGCCACCGATATTCTCAGAGCAAGGCGAGCTACCGGATTAATAAATATTTA
A

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10792.47 Da
• Isoelectric Point: 11.2088

>AT153189 WP_167810236.1 NZ_CP050813:15220-15516 [Yokenella regensburgei]
MKNVYPVKTLNQLRPLLIGFRKANALTQKDVSERLGVTQQTYARLEANPASASIERLFRVFTVLGVGISLSSGAASTAMK
PTKVKSNSEDSPARREKW

Nucleotide

• Length: 297 bp

>AT153189 NZ_CP066796:469755-470078 [Erwinia amylovora]
ATGTCAATCACACTACCTCATGAATGGGGTCTACAAAGCGATATCACCCCACGATTTGGCGCGAGATTAGTTCAGGAAGG
CAATCGGCTGTATTACCTGGCTGACAGGGCTGGATTAACGGTGAGCTTTAGCCCGATACACCTGCAGAAACTGGATCAGG
CTTTCCCGCTGTTTGTAGAACAACTGGAAAATATGCTGCGTGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTACGAGTTTAACCTGTATAGCTGATACCCGTGGTAGCTGCGGTTACGTTTACATTTCTATTTATCCAACCCC
ATAA