Detailed information of TA system TA153187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4842940..4843560
• Replicon: chromosome
• Accession: NZ_CP050811
• Organism: Yokenella regensburgei strain W13

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: G9Z4N2
• Locus tag: HEC60_RS23335
• Protein ID: WP_006818849.1
• Coordinates: 4842940..4843158 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HEC60_RS23340
• Protein ID: WP_167810000.1
• Coordinates: 4843186..4843560 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC60_RS23305	4839002..4839262	+	261	WP_006818843.1	type B 50S ribosomal protein L31	-
HEC60_RS23310	4839265..4839405	+	141	WP_006818844.1	type B 50S ribosomal protein L36	-
HEC60_RS23315	4839402..4840109	-	708	WP_167809998.1	GNAT family N-acetyltransferase	-
HEC60_RS23320	4840211..4841662	+	1452	WP_120817418.1	PLP-dependent aminotransferase family protein	-
HEC60_RS23325	4841637..4842107	-	471	WP_006818847.1	YlaC family protein	-
HEC60_RS23330	4842201..4842758	-	558	WP_167809999.1	maltose O-acetyltransferase	-
HEC60_RS23335	4842940..4843158	-	219	WP_006818849.1	hemolysin expression modulator Hha	Toxin
HEC60_RS23340	4843186..4843560	-	375	WP_167810000.1	Hha toxicity modulator TomB	Antitoxin
HEC60_RS23345	4844062..4847217	-	3156	WP_006818851.1	multidrug efflux RND transporter permease subunit	-
HEC60_RS23350	4847240..4848433	-	1194	WP_038254373.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T153187 WP_006818849.1 NZ_CP050811:c4843158-4842940 [Yokenella regensburgei]
MTDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T153187 NZ_CP066794:c2871189-2870953 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14427.14 Da
• Isoelectric Point: 4.8989

>AT153187 WP_167810000.1 NZ_CP050811:c4843560-4843186 [Yokenella regensburgei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWANDPTSAGNLQLNELLEHIATFALNYKIKYNEDNKLITQIDEYL
DDTFMLFSNYGINAEDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT153187 NZ_CP066794:c2871406-2871179 [Erwinia amylovora]
GTGCGGCTGGATGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Toxin

• AlphaFold DB: G9Z4N2