Detailed information of TA system TA153185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/dnstrm_HI1420(antitoxin)
• Location: 3992864..3993287
• Replicon: chromosome
• Accession: NZ_CP050811
• Organism: Yokenella regensburgei strain W13

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HEC60_RS19240
• Protein ID: WP_167809779.1
• Coordinates: 3993135..3993287 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A6H0KDE4
• Locus tag: HEC60_RS19235
• Protein ID: WP_038252853.1
• Coordinates: 3992864..3993142 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC60_RS19230	3991212..3992843	+	1632	WP_038252855.1	Na/Pi cotransporter family protein	-
HEC60_RS19235	3992864..3993142	-	279	WP_038252853.1	putative addiction module antidote protein	Antitoxin
HEC60_RS19240	3993135..3993287	-	153	WP_167809779.1	hypothetical protein	Toxin
HEC60_RS19245	3993461..3994345	+	885	WP_167809780.1	23S rRNA pseudouridine(2604) synthase RluF	-
HEC60_RS19250	3994346..3994618	-	273	WP_006816658.1	DUF3811 domain-containing protein	-
HEC60_RS19255	3994666..3996015	-	1350	WP_167809781.1	lysine-sensitive aspartokinase 3	-
HEC60_RS19260	3996307..3997956	+	1650	WP_006816660.1	glucose-6-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3992864..4004010	11146

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5892.96 Da
• Isoelectric Point: 11.2466

>T153185 WP_167809779.1 NZ_CP050811:c3993287-3993135 [Yokenella regensburgei]
MKEIAQTEVFRRWEAQLKDRRLKTIIAARLFRLAKSLANSLDLNKVLAHE

Nucleotide

• Length: 153 bp

>T153185 NZ_CP066794:1839002-1839373 [Erwinia amylovora]
ATGATTTATTTTCTATCCAGTCAAGACATCATCGGCATTCATCAGCGTATGATCGTCGCTTACGGCGGTCTTGCCGGTTA
TGCCGATCCTGGTCGGATAGAAAGTATGGCAACACGGATTCTGAACCGCCACATCTACGAAGGTGAGGATGATATCTATA
TTCTGGCAGCTGCATATCTGCTGGCCATTGCGCGCGGGCATTGTTTCAATGATGCAAATAAGAGAACCGCATTTGCTTCA
ACTGCTTTATTCCTGCGCCGTAATGGCATTCTTCTTCGCTTTTCCCCAATCCATGAACAGCTGACTGTCTCAGCAGCACA
GGGGAGCCTGGATGTGTGGCACATTGCTGAAGCACTGAAACAGTCGACATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9772.26 Da
• Isoelectric Point: 8.4702

>AT153185 WP_038252853.1 NZ_CP050811:c3993142-3992864 [Yokenella regensburgei]
MNKLTQYDPALALVNDEEAAAFMADAFETGDAAYIARALGVVARAKGMTKIAAQTGLSREQLYRSFSEKGNPTLKTTLAV
MNALGMGLTLKS

Nucleotide

• Length: 279 bp

>AT153185 NZ_CP066794:1838784-1839005 [Erwinia amylovora]
ATGCAAGCTATAAACTTTACTACCGCCCGTAACGAACTGGCATCAGTTCTTGATAGCGTTGCCAGCGGTGAGCCAGTTAT
GATCACCCGCAGATCGGCCAAATCCGTGGTTGTGGTTGATGCCGCGCAGTATGAAAAAATGCAAAAAATTCAGGCAGAAT
CGGACTTTGATTGGTTGTTTACAGAACATGGCCAGACGCTTGAAGCTCTTAAAAACCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A6H0KDE4