Detailed information of TA system TA153173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 670477..671120
• Replicon: chromosome
• Accession: NZ_CP050811
• Organism: Yokenella regensburgei strain W13

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HEC60_RS03305
• Protein ID: WP_167808702.1
• Coordinates: 670704..671120 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HEC60_RS03300
• Protein ID: WP_167808701.1
• Coordinates: 670477..670707 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC60_RS03265	666856..667077	-	222	WP_167808698.1	hypothetical protein	-
HEC60_RS03270	667353..667583	-	231	Protein_637	tyrosine-type recombinase/integrase	-
HEC60_RS03275	667781..668170	+	390	WP_167810096.1	aquaporin	-
HEC60_RS03280	668077..668319	-	243	Protein_639	PerC family transcriptional regulator	-
HEC60_RS03285	669081..669500	-	420	WP_167808699.1	hypothetical protein	-
HEC60_RS03290	669710..670027	+	318	Protein_641	tyrosine-type recombinase/integrase	-
HEC60_RS03295	670101..670358	-	258	WP_167808700.1	CcdB family protein	-
HEC60_RS03300	670477..670707	+	231	WP_167808701.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HEC60_RS03305	670704..671120	+	417	WP_167808702.1	type II toxin-antitoxin system VapC family toxin	Toxin
HEC60_RS03310	671215..672375	+	1161	WP_167808703.1	IS30 family transposase	-
HEC60_RS03315	672418..672810	-	393	WP_167808704.1	nuclear transport factor 2 family protein	-
HEC60_RS25405	673223..673675	+	453	WP_209319611.1	helix-turn-helix domain-containing protein	-
HEC60_RS03325	673973..674287	+	315	WP_167808706.1	PTS cellobiose transporter subunit IIB	-
HEC60_RS03330	674802..675386	+	585	WP_167808707.1	dihydrofolate reductase family protein	-
HEC60_RS03335	675620..676000	-	381	Protein_650	GrpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	csgG / csgF / csgE / csgD / csgB / csgA / flgB / flgC / flgE / flgF / flgG / flgH / flgI / flmH / iutA / iucC	519114..939233	420119
	-	flank	IS/Tn	-	-	671215..672375	1160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15133.54 Da
• Isoelectric Point: 6.7049

>T153173 WP_167808702.1 NZ_CP050811:670704-671120 [Yokenella regensburgei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVLLVDEFCARLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVTGLMLEDWVN

Nucleotide

• Length: 417 bp

>T153173 NZ_CP066777:3325430-3325633 [Morganella morganii]
ATGACTAAACTTGACTATCTCATGCGCTTACGTAAGTGCACTTCGATCGAAACACTGGAACGTGTCATTGAGAAGAACAA
ATATGAACTGACTGATGACGAGCTGGAGGTTTTTTATTCAGCTGCCGATCATCGTCTGGCCGAATTAACCATGAATAAAC
TGTATGACAAAATCCCGGCGTCTGTCTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8631.67 Da
• Isoelectric Point: 4.4692

>AT153173 WP_167808701.1 NZ_CP050811:670477-670707 [Yokenella regensburgei]
VRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFASLEKADPDFIAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT153173 NZ_CP066777:3325008-3325367 [Morganella morganii]
ATGGACGAGTATTCTCCACGAAATTACGATATTGCGGTACTGAAGTATCTGTGTGATGAACTTTGCCGTGAAGGCACACA
AACTCTCATCAGAAGCAATAATTTTTGGGTTAATGATTTAGCCTCAGATTCAAACTTAAAACTTAACGAATTATTAGGTT
ACATTGCTGACGTGACCTGGAGTTTTAAGATAAAACATACACAAAATAAGGATCTCATCTCATTTGTCGATGAATACATC
GATGAAACTTACGCGCTGTTTGGTCAGATGGAAGTTTCATTTAATGATGTTGAGGAATGGAAGAAGATGGCATCACTGGT
AAGCAGCATGCTGACCAGCGAAGGGCATCTGGTCCACTAA