Detailed information of TA system TA153136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 14141..14663
• Replicon: plasmid pSE95-1
• Accession: NZ_CP050717
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain SE95

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D0QMR1
• Locus tag: HEC96_RS22730
• Protein ID: WP_000220561.1
• Coordinates: 14141..14422 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: HEC96_RS22735
• Protein ID: WP_000121743.1
• Coordinates: 14412..14663 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HEC96_RS22685	9221..9925	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HEC96_RS22690	9991..10536	+	546	Protein_13	Tn3 family transposase	-
HEC96_RS22695	10572..10987	-	416	Protein_14	IS1 family transposase	-
HEC96_RS22700	11236..11637	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HEC96_RS22705	11570..11827	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HEC96_RS22710	11920..12129	-	210	Protein_17	CPBP family intramembrane metalloprotease	-
HEC96_RS22715	12247..12435	+	189	WP_000957857.1	hypothetical protein	-
HEC96_RS22720	12445..13644	+	1200	WP_072648941.1	IS91 family transposase	-
HEC96_RS22725	13810..13902	+	93	Protein_20	IS6 family transposase	-
HEC96_RS22730	14141..14422	-	282	WP_000220561.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HEC96_RS22735	14412..14663	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
HEC96_RS22740	15898..16737	+	840	WP_024129959.1	replication initiation protein	-
HEC96_RS22745	16758..17600	+	843	WP_000435064.1	replication initiation protein	-
HEC96_RS22750	17604..18050	+	447	WP_001074384.1	hypothetical protein	-
HEC96_RS22755	18118..18540	+	423	WP_179292036.1	DNA distortion polypeptide 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90315	90315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 13852.62 Da
• Isoelectric Point: 5.5500

>T153136 WP_000220561.1 NZ_CP050717:c14422-14141 [Salmonella enterica subsp. enterica serovar Enteritidis]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFF

Nucleotide

• Length: 282 bp

>T153136 NZ_CP066759:c4619697-4619383 [Escherichia coli O157:H7]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 12380.85 Da
• Isoelectric Point: 5.5500

>AT153136 WP_000121743.1 NZ_CP050717:c14663-14412 [Salmonella enterica subsp. enterica serovar Enteritidis]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFF

Nucleotide

• Length: 252 bp

>AT153136 NZ_CP066759:c4619933-4619700 [Escherichia coli O157:H7]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A3S5I302

Antitoxin

• AlphaFold DB: A0A3S5I301