Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | spoIISABC/SpoIISA-SpoIISB |
| Location | 1331506..1332422 | Replicon | chromosome |
| Accession | NZ_CP050705 | ||
| Organism | Bacillus atrophaeus strain PENSV20 | ||
Toxin (Protein)
| Gene name | spoIISA | Uniprot ID | A0A7U5BWH7 |
| Locus tag | HCU65_RS06710 | Protein ID | WP_003327423.1 |
| Coordinates | 1331676..1332422 (-) | Length | 249 a.a. |
Antitoxin (Protein)
| Gene name | spoIISB | Uniprot ID | A0A7U5D589 |
| Locus tag | HCU65_RS06705 | Protein ID | WP_013390483.1 |
| Coordinates | 1331506..1331676 (-) | Length | 57 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HCU65_RS06670 (1328261) | 1328261..1328590 | + | 330 | WP_003327431.1 | XkdW family protein | - |
| HCU65_RS06675 (1328587) | 1328587..1328751 | + | 165 | WP_061571316.1 | XkdX family protein | - |
| HCU65_RS06680 (1328828) | 1328828..1329676 | + | 849 | WP_219947409.1 | phage portal protein | - |
| HCU65_RS06685 (1329750) | 1329750..1330028 | + | 279 | WP_003327427.1 | hemolysin XhlA family protein | - |
| HCU65_RS06690 (1330041) | 1330041..1330304 | + | 264 | WP_003327426.1 | phage holin | - |
| HCU65_RS06695 (1330317) | 1330317..1331210 | + | 894 | WP_219947410.1 | N-acetylmuramoyl-L-alanine amidase | - |
| HCU65_RS06700 (1331286) | 1331286..1331408 | - | 123 | WP_010788356.1 | hypothetical protein | - |
| HCU65_RS06705 (1331506) | 1331506..1331676 | - | 171 | WP_013390483.1 | type II toxin-antitoxin system SpoIISB family antitoxin | Antitoxin |
| HCU65_RS06710 (1331676) | 1331676..1332422 | - | 747 | WP_003327423.1 | type II toxin-antitoxin system SpoIISA family toxin | Toxin |
| HCU65_RS06715 (1332531) | 1332531..1333529 | - | 999 | WP_003327422.1 | inorganic phosphate transporter | - |
| HCU65_RS06720 (1333542) | 1333542..1334159 | - | 618 | WP_003327421.1 | DUF47 domain-containing protein | - |
| HCU65_RS06725 (1334447) | 1334447..1335763 | - | 1317 | WP_003327418.1 | amino acid permease | - |
| HCU65_RS06730 (1336183) | 1336183..1337133 | + | 951 | WP_219947411.1 | ring-cleaving dioxygenase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 1299468..1354048 | 54580 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 249 a.a. Molecular weight: 29110.66 Da Isoelectric Point: 4.5650
>T153132 WP_003327423.1 NZ_CP050705:c1332422-1331676 [Bacillus atrophaeus]
MLLFFQIMVWCIMAALGLYVYAVWRFEAKVKEKMFAIRKTWYLIFVIGAMVYWTYDPSSLFREWTNYLIVAVSFALIDAF
IFLSAYVKKLAGNELETDTREILEENNEMLHVYLDKLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CEYSTQLEKDRLTEHMDDAFEVQSRLDRKDVYYDQHGKLALIPFTVQTRSYVIKLASDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGDG
MLLFFQIMVWCIMAALGLYVYAVWRFEAKVKEKMFAIRKTWYLIFVIGAMVYWTYDPSSLFREWTNYLIVAVSFALIDAF
IFLSAYVKKLAGNELETDTREILEENNEMLHVYLDKLKTYQYLLKNEPIHVYYGSIEAYAEGIDKLLKTYADKMNLTASL
CEYSTQLEKDRLTEHMDDAFEVQSRLDRKDVYYDQHGKLALIPFTVQTRSYVIKLASDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGDG
Download Length: 747 bp
>T153132 NZ_CP066759:3634267-3634653 [Escherichia coli O157:H7]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAAGCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA
Antitoxin
Download Length: 57 a.a. Molecular weight: 6639.60 Da Isoelectric Point: 11.0258
>AT153132 WP_013390483.1 NZ_CP050705:c1331676-1331506 [Bacillus atrophaeus]
MERAFQNDHSTRTAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
MERAFQNDHSTRTAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA
Download Length: 171 bp
>AT153132 NZ_CP066759:3634643-3634972 [Escherichia coli O157:H7]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U5BWH7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U5D589 |